BIOTECHNOLOGY & GENETIC ANALYSIS PRACTICAL ASSESSMENT Essay

BIOTECHNOLOGY & GENETIC ANALYSIS PRACTICAL ASSESSMENT - Essay ExampleAs most of these kind of catabolic genes found on plasmid observed bacterial population obtains from origin of river having very few plasmids. While in case of second sample which was obtained from nearby site of UWIC having tremendous number of plasmid bearing bacteria. This is mainly attributed to accumulation of pollutant along the path of river and accordingly bacterial population need to evolved catabolic capability to survive and hence more plasmid bearing bacterial population were found in second sample. Similarly most of the plasmid was found to be in size range more than 35 KB clearly indicates most of them are Conjugative plasmids as this group of plasmid has more number of genes compared to non-conjugative plasmid to carryout conjugation procedure and hence larger the size.Re-suspension solution constitutes of glucose, EDTA and Tris each have its own role. Glucose provides osmotic stress and EDTA as c helating agents which binds to heavy metals and helps in disintegration of cell wall. Tris act as buffering agent and maintains pH of over all reactions to avoid any pH dependent side reaction. In this stage cells become highly fragile and rough are break open.This solution is mixture of SDS and NaOH.